Orchids (Orchidaceae) are highly diverse plants known for their secondary metabolites. However, information on the distribution of secondary metabolites in plant tissues is still limited, especially in morpho-anatomical analysis using the SEM and TEM methods. This study analyzed the morpho-anatomical structure of Acriopsis liliifolia and Dendrobium mutabile, and examine the distribution and potential of their secondary metabolites. The methods used are macroscopic and microscopic morpho-anatomical observations, Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) analysis, and literature studies related to the distribution of secondary metabolites and potential in the pharmacological field. The results show that the morphology and anatomy of both species have special adaptations to the epiphytic environment, with certain tissue structures that support the accumulation of secondary metabolites. The distribution of secondary metabolites is concentrated in certain tissues such as epidermis, trichomes, and secretory tissues. The secondary metabolite content produced was identified as having potential as a pharmacological agent that can be further developed in its application as nature-based medicine. This study provides insight into the relationship between tissue structure and accumulation of bioactive compounds that contribute to the development of orchid utilization in the fields of pharmacy and biotechnology.