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Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz is a plant that can grow on mountain slopes on the island of Borneo and is locally known as Balik Angin. The leaves of this plant have been proven to contain phenolic and flavonoid compounds so it has the potential as a source of natural antioxidants. This study aimed to compare the maceration and soxhlet extraction of Balik Angin leaves in producing antioxidant activity and total phenolic-flavonoid contents. The leaves were extracted using methanol consisting of two different extractions (maceration and soxhlet). Quantitative analysis was determined spectrophotometrically for total phenolic contents, total flavonoid contents, and measurement of antioxidant activity was carried out by 2,2-diphenyl-1-picrylhydrazyl (DPPH), Cupric Ion Reducing Antioxidant Capacity (CUPRAC) and Ferric Reducing Antioxidant Power (FRAP) method. The results showed that the total phenolic contents of the soxhlet extract (708.807±0.399 µg GAE/mg) were found higher as compared to the macerated extract (631.131±0.529 µg GAE/mg), but the total flavonoid content of macerated extract (51.433±0,248 µg QE/mg) showed a higher value than soxhlet extract (44.946±0,518 µg QE/mg). The soxhlet extract has a more powerful DPPH scavenging (IC50 9.983 ppm) and reducing cupric ions (EC50 8.527 ppm) activities than the macerated extract (IC50 13.703 ppm and EC50 11.202 ppm) which both were included in a very strong antioxidant category. The ferric ion reduction power of the macerated extract was 1,739.2 mgAAE/g extract, while the soxhlet extract was 1,846.5 mgAAE/g extract. The conclusion is the soxhlet extraction of Balik Angin leaves methanol extract produces better antioxidant activity compared to the maceration and is correlated with their high phenolic contents but no significant difference in statistical analysis.

Alphitonia incana; Antioxidant; Maceration; Phenolic-flavonoid; Soxhlet