Skin aging is a multifactorial process involving complicated molecular pathways, often exacerbated by environmental stressors such as ultraviolet (UV) radiation. The growing interest in natural-based cosmetics has led to the exploration of bioactive plant extracts with anti-aging properties, including Curcuma longa and Glycyrrhiza glabra. However, effective skin delivery of such compounds remains a challenge. Nanoparticle-based systems, such as transfersomes and nanostructured lipid carriers (NLCs), offer promising approaches to enhance dermal penetration and bioavailability. This study aimed to evaluate the in vivo anti-aging efficacy of topical gels containing Curcuma longa and Glycyrrhiza glabra extracts loaded in transfersomes (TFS-CG) and NLC (NLC-CG) on UV-B-induced photoaging in Wistar rats. Thirty-five male rats were randomly assigned to seven groups (n=5), comprising a normal control, a UV-B-only group, and five treatment groups receiving various gel formulations, including TFS-CG gel, NLC-CG gel. TFS base gel, NLC base gel, and CG extract gel. The dorsal skin of the rats was irradiated with UV-B three times per week at gradually increasing doses, followed by topical application of the respective gels over three weeks. Skin elasticity was measured using the pinch test, while histological and immunohistochemical analyses were conducted to assess epidermal thickness, collagen density, and the expression of skin-aging biomarkers. Results demonstrated that TFS-CG and NLC-CG gels significantly improved skin appearance and elasticity, reduced epidermal thickening, and enhanced collagen fiber density. Immunohistochemical findings further confirmed elevated levels of COL-1, COL-2, COL-3, elastin, and TGF-β1, with concomitant suppression of MMP-1 expression. These findings support the potential of TFS-CG and NLC-CG gels as effective topical interventions for preventing UV-B-induced photoaging by restoring skin structural integrity and modulating key molecular targets.