USD Conference Systems, International Conference on Sustainable Natural Products in Healthcare 2025

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Effect of variations in Bajakah Tampala stem extraction on the sun protection factor (SPF) value and phenolic content
Erna Tri Wulandari

Last modified: 2025-05-25

Abstract


Bajakah Tampala stem (Spatholobus littoralis Hassk.) contains phenolic compounds that can protect the skin from UV exposure due to the presence of chromophore groups. The duration and ratio of simplicia: solvent affect the amount of phenolic content in the extract, so the right maceration time and ratio of simplicia: solvent will produce optimal phenolic compounds. This study aimed to see the effect of the ratio of simplicia: solvent and the duration of extraction on the total phenolic content and sunscreen activity measured in the Sun Protection Factor (SPF) value of Bajakah Tampala stem extract. Bajakah tampala stem powder was extracted by the maceration method using 96% ethanol solvent with a ratio of simplicia: solvent 1:10 b/v, 1:20 b/v, 1:30 b/v, with an extraction time of 24 hours, 48 hours, and 72 hours followed by testing sunscreen activity in vitro using UV-Vis (Ultraviolet-Visible) spectrophotometry to determine the SPF value and the Folin-Ciocalteu method was used for quantitative analysis of total phenolics. Total phenolic content is expressed as the equivalent mass value of gallic acid per g of extract. The results showed that the maceration time and the ratio of the simplicia to the solvent did not significantly affect the SPF value at concentrations of 500 ppm, 1000 ppm, and 5000 ppm. On the other hand, the length of maceration time and the ratio of the simplicia to the solvent significantly affected the total phenolic content of the ethanol extract of the Bajakah Tampala stem

Keywords


Bajakah Tampala; Phenolic; Sun Protection Factor (SPF)