Lime leaves contain flavonoids, saponins, steroids, alkaloids and tannins. The constituent often found are flavonoid compounds. This study aims to compare the total flavonoid content of lime leaf extract from four extraction methods and solvent variations. Extraction was carried out using maceration, percolation, soxhlet, and reflux methods using 96% ethanol and methanol solvents. Determination of total flavonoid content by spectrophotometric using AlCl₃ reagent at λ=429.50 nm with an operating time 30 minutes. Quercetin used as standard with concentration series of 2, 4, 6, 8, 10 and 12 μg/mL. Total flavonoid content of ethanol extract from four extraction methods analyzed statistically using Kruskal-Wallis followed by Mann-Whitney. Meanwhile, the methanol extract was analyzed statistically using One Way Anova followed by Bonferroni. The total flavonoid content of two extracts with the same extraction method were analysis by T-Independent at a 95% confidence level. The results showed that total flavonoid content of ethanol extract (maceration, percolation, soxhlet and reflux) were 0.61; 0.93; 2.05 and 2.88 mgQE/gram extract. The total flavonoid content of methanol extract were 0.76; 1.25; 2.14 and 3.82 mgQE/gram extract. Statistical results showed that total flavonoid content of ethanol extract and methanol extract were significantly different in all extraction methods.