Curcumin is a secondary metabolite known for its diverse pharmacological properties. Its concentration in turmeric can vary depending on environmental conditions. Tissue culture provides a method to grow plants more rapidly under controlled, homogeneous conditions using in vitro growth regulators. This study aimed to characterize the curcumin content in turmeric plants from Bogor and Sumedang, cultivated through tissue culture using Murashige-Skoog (MS) and Multiplication MS media. Meristematic shoots from turmeric rhizomes were cultured on MS and Multiplication MS media, the latter containing auxin and cytokinin. Explants that developed into plantlets or remained uncontaminated were subcultured for three months. The resulting plant material was extracted with 96% ethanol and analyzed for curcumin content using TLC-densitometry. Results showed that Bogor turmeric plantlets had greater average biomass than those from Sumedang. Additionally, Bogor turmeric exhibited higher total curcuminoid content. Distinct differences were observed in the levels of curcumin, demethoxycurcumin, and bisdemethoxycurcumin between MS and Multiplication MS media. Notably, the presence of growth regulators in the Multiplication MS medium led to a reduction in curcuminoid levels. Total curcuminoid content decreased by 1.5-fold in Bogor turmeric and by 3.7-fold in Sumedang turmeric when grown on Multiplication MS medium compared to standard MS.