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Breast cancer is one of the leading causes of death among women worldwide, with Estrogen Receptor Alpha-positive (ERα+)subtypes being the most prevalent. Chalcone derivatives are known for their broad biological activities, including their potential as anticancer agents. Structural modification of chalcones through the addition of alkoxy groups has been shown to enhance antiproliferative activity, particularly against breast cancer cells. This study aims to synthesis and evaluate the anticancer activity  of the chalcone derivative 1-(benzo[d][1,3]dioxol-5-yl)-3-(4-(diethoxymethyl)cyclohexa-2,4-dien-1-yl)prop-2-en-1-one against MCF-7 breast cancer cells using in vitro the MTT assay method. The synthesis was carried out using the Claisen-Schmidt Condensation method with 40% NaOH catalyst and reacted at room temperature. The result of the synthesis is a pale yellow solid with a yield percentage of 79.80%. The anticancer activity  results showed that 1-(benzo[d][1,3]dioxol-5-yl)-3-(4(diethoxymethyl)cyclohexa-2,4-dien-1-yl)prop-2-en-1-one exhibited strong cytotoxic activity against MCF-7 cells, with an IC₅₀ value of 6.286 µg/mL, and a high selectivity Index (SI) with an SI value ≥ 3 (8.314), indicating favorable selectivity towards cancer cells over normal cells. Therefore, this compound shows potential to be developed as a selective and safer alternative candidate for breast cancer.

chalcone, claisen-schmidt condensation, MCF-7 cell line, MTT assay, selectivity index