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This study evaluated the antioxidant activity and tannin content of Ocimum basilicum L. extracts from six Central Java regions (Klaten, Kudus, Pekalongan, Bandungan, Banjarnegara, and Magelang). Leaves were extracted using 96% ethanol via maceration. Antioxidant capacity was determined through the DPPH radical scavenging assay, while tannin content was quantified using the Folin-Ciocalteu method with tannic acid as a standard. Kudus samples exhibited the most vigorous activity (IC₅₀ = 56.157 ± 0.05 μg/mL), being approximately 1.6 times more potent than Banjarnegara samples (IC₅₀ = 91.279 ± 0.10 μg/mL). Bandungan showed intermediate activity (IC₅₀ = 77.722 ± 5.88 μg/mL). Regarding tannin content, Banjarnegara contained the highest levels (42.75 ± 0.5 μg/mL), followed by Bandungan (26.5 ± 0.25 μg/mL), while Klaten, Pekalongan, and Magelang displayed similar tannin concentrations (23.41-23.45 μg/mL). Notably, Kudus samples demonstrated superior antioxidant activity despite not having the highest tannin content, suggesting the involvement of other antioxidant compounds. These findings highlight the geographical factors’ influence on basil’s phytochemical properties. The study provides valuable insights for selecting optimal basil sources for nutraceutical applications, particularly emphasizing Kudus-grown basil’s exceptional antioxidant potential.

Geographical variation; Phytochemical analysis; Ocimum basilicum; DPPH; Folin-Ciocalteu