Previous studies have shown that Coleus atropurpureus leaves have analgesic effects in female mice at a dose of 252 mg/kg BW. However, the effectiveness of analgesic compounds may decrease due to interactions with food in the stomach. This study is a true experimental research with a completely randomized one-way design, aimed at investigating the effect of gastric emptying time on the analgesic response of the n-hexane fraction of ethanol extract from Coleus atropurpureus leaves (NHFCL). The test groups included a positive control (aspirin 91 mg/kg BW), a negative control (1% CMC-Na 333.3 mg/kg BW), and a treatment group (NHFCL 252 mg/kg BW). Each group was split into two subgroups, with one receiving the treatment on an empty stomach and the other receiving it 10 minutes after feeding. The pain response was measured by counting writhes induced by intraperitoneal injection of acetic acid, recorded every 5 minutes for 60 minutes, and the percentage of protection was calculated. Data were tested for normality using the Shapiro-Wilk test, followed by One-Way ANOVA and Tamhane’s Test. The results showed that gastric emptying time significantly influenced the analgesic effect of NHFCL, with a p-value <0.05 between mice treated before and after feeding.