POSTER PRESENTATION

Exploration of Endophytic Fungi from Diamond Flower Plant (Hedyotis corymbosa L. Lamk) as Candidates for New Antibacterial Agent Production

Inherni Marti Abna^1,3*, Marlia Singgih¹, Irda Fidrianny¹, Andria Agusta²

¹School of Pharmacy, Bandung Institute of Technology, Jl. Ganesa No.10, Bandung, West Java 40132, Indonesia

²Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), West Java, Indonesia

³Department of Pharmacy, Faculty of Health Sciences, Universitas Esa Unggul, Jl. Arjuna Utara No.9, Duri Kepa, Kec. Kb. Jeruk, West Jakarta City, Special Capital Region of Jakarta 11510, Indonesia

*Presenting author: reni772019@gmail.com

Infectious diseases remain a major challenge in the field of public health, particularly in developing countries like Indonesia, where environmental conditions and human activities contribute to their spread. The increasing resistance to antimicrobials poses a significant barrier to the treatment of infections, necessitating the exploration of new sources of antimicrobial agents. One plant of interest is the diamond flower plant (Hedyotis corymbosa), known for its health benefits in traditional medicine, including anticancer and antibacterial properties. This study aims to explore the bioproduction of antimicrobial agent from endophytic fungi associated with diamond flower plant. The research process includes the isolation of endophytic fungi, characterization, fermentation, extraction, and antibacterial activity testing. A total of 43 endophytic fungal isolates were successfully obtained from the roots, stems, and leaves of the diamond flower plant. After undergoing fermentation in Potato Dextrose Broth for 21 days, extraction was performed using ethyl acetate. TLC-bioautography tests identified 25 extracts that exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. The minimum inhibitory concentration (MIC) values showed that six extracts were effective against S. aureus, with the best MIC value of 64 µg/mL, and five extracts were active against E. coli with the best MIC of 128 µg/mL.

Keywords: Hedyotis corymbosa ; endophyte ; fungi; antibacterial