Last modified: 2025-06-07
Abstract
Antidepressant Activity of Pineapple (Ananas comosus L.) Juice in a Mice Model
Lively Sergeonery*, Lia Amalia, Kusnandar Anggadiredja
Department of Pharmacology School of Pharmacy, Institute Technology Bandung
* Correspondence: kusnandar_a@itb.ac.id
ABSTRACT
Introduction:
Mental health, particularly depression, has become a critical issue in achieving the sustainability goals outlined in the Sustainable Development Goals (SDGs) Agenda. Depression is associated with alterations in neurotransmission, particularly involving serotonin levels in the brain. The high prevalence of depressive disorders globally, including in Indonesia, demands urgent attention due to its profound impact on quality of life and increased risk of suicide. This study aimed to investigate the antidepressant activity of pineapple (Ananas comosus L.) juice in female BALB/c mice.
Methods:
Plant samples were obtained from cayenne-variety pineapple cultivators in Jalancagak, Subang Regency, and taxonomically identified at the Herbarium Bandungense, SITH ITB. The juice was prepared by blending fresh pineapples and extracting and freeze-drying the liquid. Organoleptic evaluations and standardization tests were performed on the juice, including water-soluble and ethanol-soluble extract contents, moisture content, total ash, acid-insoluble ash, and drying loss. Female mice aged 6–8 weeks were divided into five groups and administered either pineapple juice at 5%, 10%, or 20% b/v concentrations, fluoxetine (20 mg/kg BW) as the reference drug, or a control treatment. Treatments were given daily for 14 days. Behavioral assessments were conducted using the Forced Swimming (FST) and Open Field (OFT). Following behavioral observations, cortisol levels in the hippocampus and prefrontal cortex were measured (Figure 1).