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Dunaliela salina is a green microalgae as a source of carotenoids. The cultivation of D. salina needs to be started to provide alternative raw materials for medicines in Indonesia. D. salina has been cultured using two media for 27 days. The logarithmic growth curve shows that in both media, D. salina experiences an initiation phase (0-3 days), exponential (4-15), and stationary (after the 15th day). D. salina in M1 media experienced a growth rate (µ) of 0.145x10⁴ cells/day, doubling time (td) of 4.78 days, and increase in biomass of 0.191 mg/day. As for W2, µ = 0.195x10⁴ cells/day, td = 3.55 days, and an increase in biomass of 0.166 mg/day. D. salina in W1 and W2 produced the highest chlorophyll and carotenoids on the 24^th day, including chl-a 22.164+4.24 µg/mL, chl-b 8.93+0.66 µg/mL, and carotenoids 9.827+1.77 µg/mL for W1, and chl-a 26.962+0.25 µg/mL, chl-b 10.66+0.62 µg/mL, and carotenoids 10.961+0.07 µg/mL for W2. LCMS showed that D. salina in M1 produced 28 compounds; the dominant compound had a BM of 375.52 Da and a predicted RM of C₂₃H₃₅O₄. As for M2, there are 24 compounds; the dominant compound has a BM of 592.676 Da and a predicted RM of C₄₀H₅₂O₄.

D. salina; Carotenoids; Chlorophyl; Cultivation; microalgae